Journal:
Article Title: Internal Trehalose Protects Endocytosis from Inhibition by Ethanol in Saccharomyces cerevisiae
doi:
Figure Lengend Snippet: Tolerance to ethanol of endocytosis of the maltose transporter in growing cells. Strain ATCC 42407 grown in rich medium containing 2% glucose (A and D), 2% glucose plus 2% ethanol (B and E), or 2% ethanol (C and F) was harvested, suspended in ammonium-free medium containing 2% glucose in the absence (○, ●) or presence of 6% ethanol (▿, ▾), and incubated at 30°C. At the indicated times, measurements of transport activity (open symbols) (A through C) and immunoblots of the transporter using purified plasma membrane preparations (D through F) were performed. The intensity of the transporter band was plotted in arbitrary units (dark symbols) (A through C). The experiment was repeated three times. Differences between the values were <10%. Representative data from one experiment are shown.
Article Snippet: Strain ATCC 42407 grown in rich medium containing 2% glucose (A and D), 2% glucose plus 2% ethanol (B and E), or 2% ethanol (C and F) was harvested, suspended in ammonium-free medium containing 2% glucose in the absence (○, ●) or presence of 6% ethanol (▿, ▾), and incubated at 30°C.
Techniques: Incubation, Activity Assay, Western Blot, Purification